Description:4DNESY7MOKC6: Multiplexed FISH of AAVS1 locus in HEK293T - rTetR-KRAB (wildtype) with doxycyline treatment for 5 days + 22 days washout (mCitrine reporter OFF) Multiplexed FISH in HEK293T reporter cell line treated with doxycyline for 5 days causing reporter gene silencing followed by doxycycline wash-out for 22 days where reporter gene expression remains irreverisbly silenced, originally caused by 5-day recruitment of wt-KRAB (control and experiment relationships are described in detailed in 4DNFIWQ9QBOB supplementary file).