Description:Postprandial metabolism is a complex and dynamic physiological process involving numerous metabolic pathways and biomolecular interactions. It plays a critical role in the development of chronic diseases such as obesity, diabetes, and cardiovascular disease. This study employed an interventional design in human participants to systematically compare the dynamic postprandial changes in plasma multi-omics profiles following ingestion of a standard mixed meal versus four individual macronutrients (glucose, protein, butter, and olive oil). The aim is to elucidate the specific effects of different nutrients on human postprandial metabolic networks and their potential implications for health, thereby providing foundational data to support personalized nutrition strategies and disease prevention.