PR002725 (Project)

Description:This project aimed to assess plasma metabolite concentrations using LC-MS in two cohorts of rhesus macaques. The ad-libitum (AL, n = 11) cohort consisted of animals fed without restriction, while the calorie-restricted (CR, n = 6) cohort received a diet restricted by up to 30% of their baseline intake. Plasma samples were collected at three time points: uninfected baseline (labeled in the study design as Ad-libitum and CR), 14 days post-SIV infection (labelled as Ad-libitum+SIV, CR+SIV), and 11 months post-ART (labelled as Ad-libitum+SIV+ART, CR+SIV+ART). LC-MS metabolomics was performed on all six CR animals at every time point. In the AL cohort, only six of the eleven animals were profiled due to the high cost of metabolomics; these same six animals were sampled at all time points to maintain consistency and enable statistical comparisons without confounding.